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1 слайд
Photosynthesis:
Life from Light
2 слайд
Energy needs of life
All life needs a constant input of energy
Heterotrophs
get their energy from “eating others”
consumers of other organisms
consume organic molecules
Autotrophs
get their energy from “self”
get their energy from sunlight
use light energy to synthesize organic molecules
3 слайд
2005-2006
How are they connected?
glucose + oxygen carbon + water + energy
dioxide
C6H12O6
6O2
6CO2
6H2O
ATP
+
+
+
Heterotrophs
+ water + energy glucose + oxygen
carbon
dioxide
6CO2
6H2O
C6H12O6
6O2
light
energy
+
+
+
Autotrophs
making energy & organic molecules from light energy
making energy & organic molecules from ingesting organic molecules
4 слайд
Energy cycle
Photosynthesis
Cellular Respiration
glucose
O2
H2O
CO2
ATP
sun
The Great Circle
of Life!
Where’s Mufasa?
5 слайд
What does it mean to be a plant
Need to…
collect light energy
transform it into chemical energy
store light energy
in a stable form to be moved around the plant
& also saved for a rainy day
need to get building block atoms from
the environment
C,H,O,N,P,S
produce all organic molecules needed for growth
carbohydrates, proteins, lipids, nucleic acids
6 слайд
2005-2006
Plant structure
Obtaining raw materials
sunlight
leaves = solar collectors
CO2
stomates = gas exchange regulation
Found under leaves
H2O
uptake from roots
nutrients
uptake from roots
7 слайд
Chloroplasts
double membrane
stroma
thylakoid sacs
grana stacks
Chlorophyll & ETC in thylakoid membrane
H+ gradient built up within thylakoid sac
Plant structure
H+
H+
H+
H+
H+
H+
H+
H+
H+
H+
H+
8 слайд
2005-2006
Pigments of photosynthesis
chlorophyll & accessory pigments
“photosystem”
embedded in thylakoid membrane
structure function
Why does this structure make sense?
9 слайд
Light: absorption spectra
Photosynthesis performs work only with absorbed wavelengths of light
chlorophyll a — the dominant pigment — absorbs best in red & blue wavelengths & least in green
other pigments with different structures have different absorption spectra
10 слайд
Photosystems
Photosystems
collections of chlorophyll molecules
2 photosystems in thylakoid membrane
act as light-gathering “antenna complex”
Photosystem II
chlorophyll a
P680 = absorbs 680nm
wavelength red light
Photosystem I
chlorophyll b
P700 = absorbs 700nm
wavelength red light
11 слайд
Transfer of Electrons in PSII & PSI
In PSII, the energy from the excited e- pumps H+ into the thylakoid as it moves through the ETC.
Electrons from PSII are transferred to PSI.
After electrons have moved through PSI, an intermediary molecule (embedded in the membrane and adjacent to PSI) transfers the e- to NADP+. A H+ is attracted to this molecule and NADPH is formed.
2005-2006
12 слайд
2005-2006
Chemiosmosis in Photosynthesis
**(similar in Cell Respiration)
proton (H+)
gradient across
inner membrane
drive ATP formation
ATP synthase
enzyme
13 слайд
Summary of the LDR
PS II absorbs light
excited electron passes from chlorophyll to “primary electron acceptor” at the REACTION CENTER.
splits H2O (Photolysis!!)
O2 released to atmosphere
PS I absorbs light
Produces NADPH (stored energy) which will be used by the Calvin cycle
Chemiosmosis produces ATP from light energy
ATP will be used by the Calvin Cycle
14 слайд
2005-2006
ETC of Photosynthesis
Chloroplasts transform light energy into chemical energy of ATP
use electron carrier NADPH
split H2O
15 слайд
2005-2006
2 Photosystems
Light reactions elevate electrons in
2 steps (PS II & PS I)
PS II helps generate
energy as ATP (H+ pumps)
PS I generates
reducing power as NADPH
This shows Noncyclic photophosphorylation.
16 слайд
ETC of Photosynthesis
17 слайд
ETC of Photosynthesis
18 слайд
2005-2006
Cyclic photophosphorylation
If PS I can’t pass electron to NADP, it cycles back to PS II & makes more ATP, but no NADPH
coordinates light reactions to Calvin cycle
Calvin cycle uses more ATP than NADPH
19 слайд
Do Now: Light Reactions Summary Questions
Where did the energy come from?
Where did the H2O come from?
Where did the electrons come from?
Where did the O2 come from?
Where did the H+ come from?
Where did the ATP come from?
Where did the O2 go?
What will the ATP be used for?
What will the NADPH be used for?
20 слайд
Photosynthesis overview
Light reactions
convert solar energy to chemical energy
ATP
Calvin cycle
uses chemical
energy (NADPH & ATP)
to reduce CO2 to
build C6H12O6 (sugars)
21 слайд
2005-2006
From Light reactions to Calvin cycle
Calvin cycle
Chloroplast stroma
Need products of light reactions to drive synthesis reactions
ATP
NADPH
22 слайд
From CO2 C6H12O6
CO2 has very little chemical energy
fully oxidized
C6H12O6 contains a lot of chemical energy
reduced
endergonic
Reduction of CO2 C6H12O6 proceeds in many small uphill steps
each catalyzed by specific enzyme
using energy stored in ATP & NADPH
23 слайд
PGAL
to make
glucose
6C
unstable
intermediate
1C
CO2
Calvin cycle
5C
RuBP
3C
2x
PGA/GP
6 ADP
6 ATP
3C
2x
3C
x2
PGAL/G3P
6 NADP
6 NADPH
3 ADP
3 ATP
Rubisco
1. Carbon fixation
2. Reduction
3. Regeneration
ribulose bisphosphate
ribulose
bisphosphate
carboxylase
sucrose
cellulose
etc.
24 слайд
Rubisco
Enzyme which fixes carbon from atmosphere
ribulose bisphosphate carboxylase
the most important enzyme in the world!
it makes life out of air!
definitely the most abundant enzyme
25 слайд
Calvin cycle
PGAL/G3P
end product of Calvin cycle
energy rich sugar
3 carbon compound
“C3 photosynthesis”
PGAL/G3P important intermediate
PGAL glucose carbohydrates
lipids
amino acids
nucleic acids
26 слайд
2005-2006
Photosynthesis summary
Light reactions
produced ATP
produced NADPH
consumed H2O
produced O2 as byproduct
Calvin cycle
consumed CO2
produced PGAL/G3P
regenerated ADP
regenerated NADP
27 слайд
2005-2006
Summary of photosynthesis
Where did the CO2 come from?
Where did the CO2 go?
Where did the H2O come from?
Where did the H2O go?
Where did the energy come from?
What’s the energy used for?
What will the C6H12O6 be used for?
Where did the O2 come from?
Where will the O2 go?
What else is involved that is not listed in this equation?
6CO2
6H2O
C6H12O6
6O2
light
energy
+
+
+
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